[1]李 曼,石兴民,郭 睿,等.胸腹水中肿瘤相关巨噬细胞不同活化亚型的分析[J].国外医学医学地理杂志,2019,(02):120-123.[doi:10.3969/j.issn.1001-8883.2019.02.006]
 LI Man,SHI Xingmin,GUO Rui,et al.Analysis of different activation subtypes of tumor-associatedmacrophages in pleural and ascites[J].Foreign Medical Sciences (Section of Medgeography),2019,(02):120-123.[doi:10.3969/j.issn.1001-8883.2019.02.006]
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胸腹水中肿瘤相关巨噬细胞不同活化亚型的分析()
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国外医学医学地理杂志[ISSN:1001-8883/CN:61-1102/R]

卷:
期数:
2019年02期
页码:
120-123
栏目:
基础医学
出版日期:
2019-07-02

文章信息/Info

Title:
Analysis of different activation subtypes of tumor-associatedmacrophages in pleural and ascites
作者:
李 曼1石兴民2郭 睿1杨 军1
(1.西安交通大学医学院第二附属医院,陕西西安 710049; 2.西安交通大学医学部公共卫生学院,陕西西安 710004)
Author(s):
LI Man1SHI Xingmin2GUO Rui1YANG Jun1
(1.The Second Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710049; 2.School of Public Health,Medical School of Xi’an Jiaotong University,Xi’an 710004,China)
关键词:
胸腹水 细胞蜡块 免疫组织化学标记 巨噬细胞
Keywords:
pleural and ascites cell wax block immune histochemical markers macrophages
分类号:
R153
DOI:
10.3969/j.issn.1001-8883.2019.02.006
文献标志码:
A
摘要:
目的 研究不同性质胸腹水中肿瘤相关巨噬细胞表型的特异性标志物CD68、CD86、CD163来判断巨噬细胞的类型,并通过对肿瘤相关巨噬细胞浸润及相关分型和主要功能的研究,探讨其临床意义。方法 胸腹水细胞蜡块标本76例,其中恶性肺癌标本59例,良性标本17例,通过免疫组化的方法检测巨噬细胞表型的特异性标志物CD68、CD86、CD163在其中的表达水平。结果 巨噬细胞表型的特异性标志物CD68、CD86、CD163在良性组的染色强度、阳性表达比肺癌组均较低,差异具有统计学意义(P<0.05)。肺癌标本中M1、M2型巨噬细胞的比例较良性病变患者差异均无统计学意义(P>0.05)。结论 胸腹水中肿瘤相关巨噬细胞表型的特异性标志物CD68、CD86、CD163在肺癌组中表达水平均显著升高,可为临床诊断肺癌提供重要参考。
Abstract:
Objective To investigate the specific markers of tumor-associated macrophage phenotypes in different types of pleural and ascites,such as CD68,CD86 and CD163,to determine the type of macrophages,and to investigate tumor-associated macrophage infiltration and related typing and main functions.Methods The study explores its clinical implications.Methods seventy-seven specimens of sacral and ascites cells were obtained,including 59 cases of malignancy and 17 cases of benign.The expression levels of CD68,CD86 and CD163 markers were detected by immunohistochemistry.Results The staining intensity and positive expression of macrophage phenotype specific markers CD68、CD86 and CD163 in benign group were lower than those in lung cancer group,and the difference was statistically significant(P<0.05).There was no statistically significant difference in the proportion of M1 and M2 macrophages in lung cancer specimens than in patients with benign lesions(P>0.05).Conclusion The specific markers of tumor-related macrophage phenotype in thoracic ascites,CD68、CD86 and CD163,have increased significantly in lung cancer group,which can provide important reference for clinical diagnosis of lung cancer.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期:2018-12-29 修回日期:2019-02-11
通信作者:石兴民,男(汉族),副教授,硕士生导师.
作者简介:李曼(1990-),女(汉族),在读硕士.研究方向:病理学与免疫组化.
更新日期/Last Update: 2019-07-02