[1]曹国芬,孟改利,张永爱.围绝经期小鼠抑郁样行为及相关脑区GABAA受体的改变[J].国外医学医学地理杂志,2019,(02):126-130.[doi:10.3969/j.issn.1001-8883.2019.02.008]
　CAO Guofen,MENG Gaili,ZHANG Yongai.Changes of depressive-like behavior and GABAA receptors inrelated brain regions in perimenopausal mice[J].Foreign Medical Sciences (Section of Medgeography),2019,(02):126-130.[doi:10.3969/j.issn.1001-8883.2019.02.008]
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围绝经期小鼠抑郁样行为及相关脑区GABAA受体的改变()
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国外医学医学地理杂志[ISSN:1001-8883/CN:61-1102/R]

卷:
期数:
2019年02期
页码:
126-130
栏目:
基础医学
出版日期:
2019-07-02

文章信息/Info

Title:
Changes of depressive-like behavior and GABAA receptors inrelated brain regions in perimenopausal mice
作者:
曹国芬1; 孟改利2; 张永爱1
(1.西安医学院护理学院,陕西西安 710021; 2.西北妇女儿童医院,陕西西安 710061)
Author(s):
CAO Guofen1; MENG Gaili2; ZHANG Yongai1
(1.Xi’an Medical University School of Nursing,Xi’an 710021; 2.Northwest Women and Children Hospital,Xi’an 710061,China)
关键词:
围绝经期;  抑郁样行为;  Gabra1;  Gabrb2;  Gabrg2;  海马;  前额叶皮质
Keywords:
perimenopause;  depressive-like behavior;  Gabra1;  Gabrb2;  Gabrg2;  hippocampus;  prefrontal cortex
分类号:
R339.3
DOI:
10.3969/j.issn.1001-8883.2019.02.008
文献标志码:
A
摘要:
目的 研究围绝经期小鼠抑郁样行为及相关脑区GABAA受体的改变。方法 采用2、8、12 m雌性C57BL/6小鼠,采用悬尾实验、强迫游泳实验检测小鼠不动时间的变化,研究小鼠抑郁样行为的改变。进一步,采用实时定量PCR方法检测海马,前额叶皮质中GABAA受体Gabra1、Gabrb2及Gabrg2的mRNA改变。结果 三组小鼠在悬尾实验、强迫游泳实验中不动时间无明显差异(P>0.05); 海马、前额叶皮质中Gabra1、Gabrb2在12 m组小鼠明显降低(P<0.05); 海马中Gabra1水平在3组之间呈逐渐下降,而前额叶皮质中Gabra1,海马、前额叶皮质中Gabrb2在8 m组呈一定程度上升,至12 m时明显下降(P<0.05); 海马及前额叶皮质中Gabrg2在3组之间无明显差异(P>0.05)。结论 无应激状态下,围绝经期小鼠抑郁样行为无明显变化; 小鼠海马及前额叶皮质中Gabrg2可能参与该行为过程; 海马、前额叶皮质中Gabra1,Gabrb2在围绝经期明显下降,可能参与应激诱导的围绝经期抑郁易感性。
Abstract:
Objective To study changes of depressive-like behaviors and GABAA receptors in related brain regions in perimenopausal mice.Methods Female C57BL/6 mice of 2 m,8 m and 12 m were used in the current study.The immobility time in tail suspension test(TST)and forced swim test(FST)were detected to evaluate the changes of depressive-like behavior in mice.Furthermore,real-time quantitative PCR was used to detect mRNA changes of GABAA receptors Gabra1,Gabrb2 and Gabrg2 in the hippocampus and prefrontal cortex.Results There was no significant difference in the immobility time among the three groups of mice in the TST and FST(P>0.05).The Gabra1 as well as Gabrb2 in the hippocampus and prefrontal cortex were significantly decreased in the 12 m group(P<0.05); the mRNA level of Gabra1 decreased gradually with aging of the three groups,while Gabra1 in the prefrontal cortex,Gabrb2 in the hippocampus and prefrontal cortex increased to a certain extent in the 8 m group,and then decreased significantly at 12 m(P<0.05).There was no significant difference in Gabrg2 among the three groups in the hippocampus and prefrontal cortex(P>0.05).Conclusion There was no significant change in depressive-like behavior in perimenopausal mice without stress; Gabrg2 in the hippocampus and prefrontal cortex might be involved in this behavioral process in mice; Gabra1 and Gabrb2 in the hippocampus and prefrontal cortex were significantly decreased during perimenopause,which might be involved in stress-induced perimenopausal depression susceptibility.

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备注/Memo

备注/Memo:
收稿日期:2019-01-10 修回日期:2019-03-15
基金项目:陕西省教育厅专项科研计划项目(No.17JK0657); 西安医学院护理学省级重点学科建设项目(No.16hlxk16); 西安医学院博士科研启动基金项目(No.2015DOC22)
作者简介:曹国芬(1985-),女(汉族),博士,讲师.研究方向:抑郁症的神经生物学机制研究.
更新日期/Last Update: 2019-07-02